中文名 | ITSA-1 |
英文名 | Benzotriazol-1-yl-(2,4-Dichloro-Phenyl)-Methanone |
别名 | 化合物ITSA1 (1H-苯并[D][1,2,3]三唑-1-基)(2,4-二氯苯基)甲酮 |
英文别名 | ITSA-1 CS-2249 ITSA-1(ITSA1) IFLAB-BB F0451-3184 1-(2,4-Dichlorobenzoyl)-1H-benzotriazole 1-(2,4-DICHLOROBENZOYL)-1H-BENZOTRIAZOLE Benzotriazol-1-yl-(2,4-Dichloro-Phenyl)-Methanone BENZOTRIAZOL-1-YL-(2,4-DICHLORO-PHENYL)-METHANONE Methanone, 1H-benzotriazol-1-yl(2,4-dichlorophenyl)- (1H-Benzo[d][1,2,3]triazol-1-yl)(2,4-dichlorophenyl)methanone |
CAS | 200626-61-5 |
化学式 | C13H7Cl2N3O |
分子量 | 292.12 |
熔点 | 119-121°C |
溶解度 | DMSO: ~ 28 mg/mL |
存储条件 | Inert atmosphere,2-8°C |
外观 | 固体 |
颜色 | White to Off-White |
体外研究 | ITSA1 (50 μM; A549 cells) treatment serves to revert the TSA-arrested population to a normal cell cycle distribution. ITSA1 is also able to effect cell cycle rescue over longer duration. ITSA1 (50 μM; 5 hours; A549 cells) treatment reduces the number of apoptosis in TSA-treated cells. ITSA1 (50 μM; 2 hours; A549 and murine ES cells cells) treatment suppresses TSA-induced histone acetylation. Importantly, suppression of acetylation levels is only observable when ITSA1 is added concurrent with or post TSA treatment. ITSA1 (50 μM; 30 minutes; murine ES cells cells) suppresses TSA-activated transcription in murine ES cells. Cell Cycle Analysis Cell Line: Murine ES cells Concentration: 50 μM Incubation Time: Result: Served to revert the TSA-arrested population to a normal cell cycle distribution. Apoptosis Analysis Cell Line: A549 cells Concentration: 50 μM Incubation Time: 5 hours Result: Reduced the number of apoptosis. Western Blot Analysis Cell Line: A549 and murine ES cells Concentration: 50 μM Incubation Time: 2 hours Result: Reduced histone acetylation to the baseline level. RT-PCR Cell Line: Murine ES cells Concentration: 50 μM Incubation Time: 30 minutes Result: Suppressed TSA-activated transcription. |
体内研究 | ITSA-1 (0.5 mg/kg; intraperitoneal injection; 3 times/week; for 8 weeks; CBS +/− mice) treatment balances deacetylation activity and suppresses IL-6 and TNF-α expression and thereby attenuated histone acetylationdependent infammatory signaling. Animal Model: CBS +/− mice Dosage: 0.5 mg/kg Administration: Intraperitoneal injection; 3 times/week; for 8 weeks Result: Balanced deacetylation activity and suppressed IL-6 and TNF-α expression. |
危险品标志 | Xn - 有害物品 |
风险术语 | 22 - 吞食有害。 |
WGK Germany | 3 |
1mg | 5mg | 10mg | |
---|---|---|---|
1 mM | 3.423 ml | 17.116 ml | 34.233 ml |
5 mM | 0.685 ml | 3.423 ml | 6.847 ml |
10 mM | 0.342 ml | 1.712 ml | 3.423 ml |
5 mM | 0.068 ml | 0.342 ml | 0.685 ml |
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